WP 10 Indoor factors and health impacts in adults
Participating partners: THL, UU, CREAL, HMGU, IMPERIAL
This WP aims to provide systematic information about indoor endotoxin, and other microbial components across Europe, factors that contribute to the development of avoidance measures of indoor air pollution, and to assess the long-term health effect of exposure to these indoor factors. The WP will combine this exposure information with the previously collected highly standardized health data of the European Community Respiratory Health Survey (ECRHS).
The geographical variation of the distributions of indoor endotoxin and microbial components will be analysed. At stage 1, microbial groups and/or species will be quantified in 400 dust samples via QPCR. In addition, DNA fingerprinting technique will be applied to explore differences in microbial communities in these 400 home dust samples (WP 5). At stage 2, indoor endotoxin and the most promising and efficient microbial groups selected in stage 1 will be carried out in the 1000 stored settled house dust sampled from 22 centres of the ECRHS II (WP 5 and 7). The determinants for the geographical variation of the distributions of indoor endotoxin and 2 to 3 microbial components and factors that contribute to the development of avoidance measures will be identified.
The long term effect of exposure to indoor endotoxin and microbial components on lung function, bronchial hyperactivity, and respiratory symptoms including atopic and non-atopic asthma in adults will be assessed.
This WP aims to provide systematic information about indoor endotoxin, and other microbial components across Europe, factors that contribute to the development of avoidance measures of indoor air pollution, and to assess the long-term health effect of exposure to these indoor factors. The WP will combine this exposure information with the previously collected highly standardized health data of the European Community Respiratory Health Survey (ECRHS).
The geographical variation of the distributions of indoor endotoxin and microbial components will be analysed. At stage 1, microbial groups and/or species will be quantified in 400 dust samples via QPCR. In addition, DNA fingerprinting technique will be applied to explore differences in microbial communities in these 400 home dust samples (WP 5). At stage 2, indoor endotoxin and the most promising and efficient microbial groups selected in stage 1 will be carried out in the 1000 stored settled house dust sampled from 22 centres of the ECRHS II (WP 5 and 7). The determinants for the geographical variation of the distributions of indoor endotoxin and 2 to 3 microbial components and factors that contribute to the development of avoidance measures will be identified.
The long term effect of exposure to indoor endotoxin and microbial components on lung function, bronchial hyperactivity, and respiratory symptoms including atopic and non-atopic asthma in adults will be assessed.